Journal: Antibiotics

Article Title: Antimicrobial and Antibiofilm Potential of Thymus vulgaris and Cymbopogon flexuosus Essential Oils against Pure and Mixed Cultures of Foodborne Bacteria

doi: 10.3390/antibiotics12030565

Figure Lengend Snippet: Growth conditions and terminology used for the strains included in this study.

Article Snippet: For L1 ( L. monocytogenes CECT 937) and A1 (A1— A. hydrophila A259), an intermediate MBEC of 443.14 μg/μL was reported, and for E2 ( E. faecalis V583) and B1 ( B. thermosphacta ATCC 11509 Τ ), it was 886.28 μg/μL.

Techniques:

Journal: Antibiotics

Article Title: Antimicrobial and Antibiofilm Potential of Thymus vulgaris and Cymbopogon flexuosus Essential Oils against Pure and Mixed Cultures of Foodborne Bacteria

doi: 10.3390/antibiotics12030565

Figure Lengend Snippet: Schematic representation of the polystyrene 96-well microtiter plates inoculated with pure bacterial suspensions, for the incubation periods of 72, 48, and 24 h at 25 °C, or 5, 12, and 16 days at 4 °C ( A ), and mixed cultures used for subsequent assays ( B ). B1— B. thermosphacta ATCC11509 T ; A1— A. hydrophila A259; E1— E. faecalis QSE123; E2— E. faecalis V583; L1— L. monocytogenes CECT 937; L2— L. monocytogenes CECT 935. Note 1: Negative control (sterile control) contained only BHI broth. Note 2: Similar organization was applied for the mixed culture assays.

Article Snippet: For L1 ( L. monocytogenes CECT 937) and A1 (A1— A. hydrophila A259), an intermediate MBEC of 443.14 μg/μL was reported, and for E2 ( E. faecalis V583) and B1 ( B. thermosphacta ATCC 11509 Τ ), it was 886.28 μg/μL.

Techniques: Incubation, Negative Control, Sterility, Control

Journal: Antibiotics

Article Title: Antimicrobial and Antibiofilm Potential of Thymus vulgaris and Cymbopogon flexuosus Essential Oils against Pure and Mixed Cultures of Foodborne Bacteria

doi: 10.3390/antibiotics12030565

Figure Lengend Snippet: Schematic organization of the 24-well plate with the pure bacterial suspensions ( A ) and mixed cultures ( B ), used in the stainless steel biofilm formation assays (4 °C for 12 days or 25 °C for 48 h). L1— L. monocytogenes CECT 937; A1— A. hydrophila A259; L2— L. monocytogenes CECT 935; B1— B. thermosphacta ATCC 11509 T ; E1— E. faecalis QSE123; E2— E. faecalis V583. Note: Negative control contained the stainless steel disk and BHI broth.

Article Snippet: For L1 ( L. monocytogenes CECT 937) and A1 (A1— A. hydrophila A259), an intermediate MBEC of 443.14 μg/μL was reported, and for E2 ( E. faecalis V583) and B1 ( B. thermosphacta ATCC 11509 Τ ), it was 886.28 μg/μL.

Techniques: Negative Control

Journal: Antibiotics

Article Title: Antimicrobial and Antibiofilm Potential of Thymus vulgaris and Cymbopogon flexuosus Essential Oils against Pure and Mixed Cultures of Foodborne Bacteria

doi: 10.3390/antibiotics12030565

Figure Lengend Snippet: Schematic organization of the polystyrene 96-well microtiter plates used for pure cultures, incubated with EOs in 1:2 ( v / v ) dilutions. B1— B. thermosphacta ATCC 11509 T ; A1— A. hydrophila A259; E1— E. faecalis QSE123; E2— E. faecalis V583; L1— L. monocytogenes CECT 937; L2— L. monocytogenes CECT 935. Note 1: Positive control contained BHI broth inoculated with the correspondent bacteria. Note 2: Negative control contained only BHI broth.

Article Snippet: For L1 ( L. monocytogenes CECT 937) and A1 (A1— A. hydrophila A259), an intermediate MBEC of 443.14 μg/μL was reported, and for E2 ( E. faecalis V583) and B1 ( B. thermosphacta ATCC 11509 Τ ), it was 886.28 μg/μL.

Techniques: Incubation, Positive Control, Bacteria, Negative Control

Journal: Antibiotics

Article Title: Antimicrobial and Antibiofilm Potential of Thymus vulgaris and Cymbopogon flexuosus Essential Oils against Pure and Mixed Cultures of Foodborne Bacteria

doi: 10.3390/antibiotics12030565

Figure Lengend Snippet: Biofilm formation by pure ( A , C ) and mixed ( B , D ) cultures under different temperatures, 4 °C for 12 days or 25 °C for 48 h, using two distinct contact surfaces (polystyrene and stainless steel). L1— L. monocytogenes CECT 937; L2— L. monocytogenes CECT 935; E1— E. faecalis QSE123; E2— E. faecalis V583; A1— A. hydrophila A259; B1— B. thermosphacta ATCC 11509 T . Note 1: Data displayed represent the average of each technical replicate and corresponding standard deviation values.

Article Snippet: For L1 ( L. monocytogenes CECT 937) and A1 (A1— A. hydrophila A259), an intermediate MBEC of 443.14 μg/μL was reported, and for E2 ( E. faecalis V583) and B1 ( B. thermosphacta ATCC 11509 Τ ), it was 886.28 μg/μL.

Techniques: Standard Deviation